THETM His Tag Antibody [Biotin], mAb, Mouse
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THE TM His Tag Antibody [Biotin], mAb, Mouse  |
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![THE<sup>TM</sup> His Tag Antibody [Biotin], mAb, Mouse](/images/cgi2007/table2.gif "THE<sup>TM</sup> His Tag Antibody [Biotin], mAb, Mouse")
| Full Name |
THETM His Tag Antibody [Biotin], mAb, Mouse
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Abbreviated name-1 |
THETM His Tag Antibody [Biotin], mAb, Mouse |
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Description |
GenScript THETM His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THETM His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of His in fusion proteins. |
Ig Subclass |
IgG1, k |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
Clone |
6G2A9 |
Immunogen |
A synthetic peptide (HHHHHH) coupled to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other applications: user-optimized |
Conjugation |
Biotin |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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Related Products
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Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
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Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
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Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
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![THE<sup>TM</sup> His Tag Antibody [Biotin], mAb, Mouse](/images/download_PDF.jpg "THE<sup>TM</sup> His Tag Antibody [Biotin], mAb, Mouse")
MSDS: 20080213024757 (PDF)![Mouse Anti His-tag [Biotin] mAb](/site2/document/3376_20070711050658.JPG "THE<sup>TM</sup> His Tag Antibody [Biotin], mAb, Mouse")
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